FC experiment of mouse splenocytes using 98427-1-TR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG2a Isotype Control RecAb (98427-1-TR, Clone: 250862A1) and FITC anti-rat IgG2a at dilution 1:100 (red), or 0.25 ug Anti-Mouse CD4 (65141-1-Ig,Clone: RM4-5) and FITC anti-rat IgG2a at dilution 1:100 (green), or unstained (blue). Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG2a Isotype Control RecAb (98427-1-TR, Clone: 250862A1) and FITC anti-rat IgG2a at dilution 1:100 (red), or 0.25 ug Anti-Mouse CD4 (65141-1-Ig,Clone: RM4-5) and FITC anti-rat IgG2a at dilution 1:100 (green), or unstained (blue). Cells were not fixed.
FC experiment of mouse splenocytes using 98427-1-TR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG2a Isotype Control RecAb (98427-1-TR, Clone: 250862A1) and FITC anti-rat IgG2a at dilution 1:100(right), or unstained (left). Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG2a Isotype Control RecAb (98427-1-TR, Clone: 250862A1) and FITC anti-rat IgG2a at dilution 1:100(right), or unstained (left). Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98427-1-TR targets IgG2a Isotype Control in FC applications and shows reactivity with n/a samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Protocols
Product Specific Protocols
FC protocol for IgG2a Isotype Control antibody 98427-1-TR
FC experiment of mouse splenocytes using 98427-1-TR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG2a Isotype Control RecAb (98427-1-TR, Clone: 250862A1) and FITC anti-rat IgG2a at dilution 1:100 (red), or 0.25 ug Anti-Mouse CD4 (65141-1-Ig,Clone: RM4-5) and FITC anti-rat IgG2a at dilution 1:100 (green), or unstained (blue). Cells were not fixed.
FC experiment of mouse splenocytes using 98427-1-TR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG2a Isotype Control RecAb (98427-1-TR, Clone: 250862A1) and FITC anti-rat IgG2a at dilution 1:100(right), or unstained (left). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgG2a Isotype Control Recombinant antibody
Catalog Number
98427-1-TR
Citations
-
Dilutions
Applications
FC
Reactivity
n/a
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
