Rat IgG2a Isotype Control Recombinant Antibody, PBS Only

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  FC experiment of mouse splenocytes using 98427-1-TR (same clone as 98427-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG2a Isotype Control RecAb (98427-1-TR, Clone: 250862A1) and FITC anti-rat IgG2a at dilution 1:100 (red), or 0.25 ug Anti-Mouse CD4 (65141-1-Ig,Clone: RM4-5) and FITC anti-rat IgG2a at dilution 1:100 (green), or unstained (blue). Cells were not fixed. This data was developed using the same antibody clone with 98427-1-PBS in a different storage buffer formulation.

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  FC experiment of mouse splenocytes using 98427-1-TR (same clone as 98427-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG2a Isotype Control RecAb (98427-1-TR, Clone: 250862A1) and FITC anti-rat IgG2a at dilution 1:100(right), or unstained (left). Cells were not fixed. This data was developed using the same antibody clone with 98427-1-PBS in a different storage buffer formulation.

IgG2a Isotype Control Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98427-1-PBS

Clone No.250862A1

Host / Isotype

Rat / IgG2a

Reactivity

n/a

Applications

FC

Synonyms

IgG2a, IgG2a Isotype, Rat IgG2a, Rat IgG2a Isotype, Rat IgG2a Isotype Control

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Product Information

98427-1-PBS targets IgG2a Isotype Control in FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Small molecule
• Full Name: IgG2a Isotype Control
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.