CoraLite® Plus 647 Rat IgG1 Isotype Control (HRPN)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

HRPN

Cat no : CL647-65212

Synonyms

IgG1, IgG1 Isotype Control, Rat IgG1 Isotype Control

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL647-65212

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite®488-conjugated Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and 5 ul CoraLite® Plus 647-conjugated Anti-Mouse CD25 (CL647-65137, Clone: PC61.5). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL647-65212

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite®488-conjugated Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) and CoraLite® Plus 647-conjugated Rat IgG1 Isotype Control (CL647-65212, Clone: HRPN). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65212 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgG1 Isotype Control
• RRID: AB_2920270
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The HRPN immunoglobulin is useful as an isotype-matched control. The HRPN immunolglobulin has an unknown binding specificity and is used as an isotype control for rat IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.