Rat IgG1 Isotype Control (HRPN)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Applications

FC

Conjugate

Unconjugated

CloneNo.

HRPN

Cat no : 65212-1-Ig

Synonyms

Rat IgG1 Isotype Control, IgG1

---

Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 65212-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG1 Isotype Control (HRPN) (65212-1-Ig, Clone: HRPN) and FITC anti-rat IgG1 Antibody (red), or 0.25 ug Rat IgG1 Isotype Control (HRPN) (65212-1-Ig, Clone: HRPN) and FITC anti-rat IgG1 Antibody (blue), or not stained (blank) (black). Cells were not fixed.

---

Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65212-1-Ig targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgG1 Isotype Control
• RRID: AB_2918473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

The HRPN immunoglobulin is useful as an isotype-matched control. The HRPN immunolglobulin has an unknown binding specificity and is used as an isotype control for rat IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.