ITCH Monoclonal antibody, PBS Only

ITCH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E3G9

Cat no : 67757-1-PBS

Synonyms

AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1

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Validation Data Gallery

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  WB analysis using 67757-1-Ig (same clone as 67757-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67757-1-Ig (same clone as 67757-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67757-1-Ig (same clone as 67757-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67757-1-Ig (same clone as 67757-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67757-1-Ig (same clone as 67757-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67757-1-Ig (same clone as 67757-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ITCH antibody (67757-1-Ig, Clone: 2E3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

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Product Information

67757-1-PBS targets ITCH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ITCH fusion protein Ag14688
• Full Name: itchy E3 ubiquitin protein ligase homolog (mouse)
• Calculated Molecular Weight: 903 aa, 103 kDa
• Observed Molecular Weight: 103 kDa, 70 kDa
• GenBank Accession Number: BC011571
• Gene Symbol: ITCH
• Gene ID (NCBI): 83737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.