ISLR2 Recombinant antibody

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  WB analysis using 84076-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84076-3-RR (ISLR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84076-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84076-3-RR against Human ISLR2 were performed. The affinity constant is below 1 pM.

ISLR2 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84076-3-RR

Clone No.241227D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

LINX, Leucine-rich repeat domain and immunoglobulin domain-containing axon extension protein, KIAA1465, Immunoglobulin superfamily containing leucine-rich repeat protein 2, 241227D6

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84076-3-RR targets ISLR2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ISLR2 fusion protein Ag20505
• Full Name: immunoglobulin superfamily containing leucine-rich repeat 2
• Calculated Molecular Weight: 745 aa, 79 kDa
• Observed Molecular Weight: 79 kDa, 130 kDa
• GenBank Accession Number: BC152429
• Gene Symbol: ISLR2
• Gene ID (NCBI): 57611
• RRID: AB_3671645
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q6UXK2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ISLR2 (immunoglobulin superfamily containing leucine rich repeat 2), also known as LINX. It is expected to be located in cell membrane and the protein is mainly expressed in brain and testis. While the first knockout mouse had impaired development of the internal capsule and no hydrocephalus, a much more recent independent knockout mouse was shown to additionally have severe hydrocephalus. Although the mechanism remains unclear, it may be related to the role of ISLR2 in reorganizing the cytoskeleton of developing neurons (PMID: 30483960). The calculated molecular weight of ISLR2 is 79 kDa, this target has phosphorylation modification and glycosylation modification. And the 130 kDa band is glycosylated (PMID: 29739947).