ISG15 Monoclonal antibody, PBS Only

ISG15 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G9A2

Cat no : 68408-1-PBS

Synonyms

G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP

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Validation Data Gallery

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  WB analysis using 68408-1-Ig (same clone as 68408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 68408-1-Ig (same clone as 68408-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68408-1-Ig (same clone as 68408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

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Product Information

68408-1-PBS targets ISG15 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ISG15 fusion protein Ag8953
• Full Name: ISG15 ubiquitin-like modifier
• Calculated Molecular Weight: 165 aa, 18 kDa
• Observed Molecular Weight: 13-17 kDa
• GenBank Accession Number: BC009507
• Gene Symbol: ISG15
• Gene ID (NCBI): 9636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.