ISG15 Monoclonal antibody

ISG15 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3G9A2

Cat no : 68408-1-Ig

Synonyms

G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP

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Validation Data Gallery

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  WB analysis using 68408-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 68408-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68408-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, LNCaP cells, A431 cells, HeLa cells, HCT 116 cells, HeLa cells, DU 145 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

68408-1-Ig targets ISG15 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ISG15 fusion protein Ag8953
• Full Name: ISG15 ubiquitin-like modifier
• Calculated Molecular Weight: 165 aa, 18 kDa
• Observed Molecular Weight: 13-17 kDa
• GenBank Accession Number: BC009507
• Gene Symbol: ISG15
• Gene ID (NCBI): 9636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.

Publications

Species	Application	Title
human	IHC	Exp Mol Pathol; The relationship between tumor immunity and the cGAS-STING pathway in breast cancer: An immunohistochemical study; Authors - Haruto Nishida