IRX6 Recombinant antibody

IRX6 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240876D11

Cat no : 83800-5-RR

Synonyms

IRXB3, IRX7, IRX 3, Iroquois-class homeodomain protein IRX-6, iroquois homeobox 6

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Validation Data Gallery

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  WB analysis using 83800-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83800-5-RR (IRX6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83800-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83800-5-RR against Human IRX6 were performed. The affinity constant is 1.56 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, NCCIT cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83800-5-RR is IRX6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IRX6 fusion protein Ag19586
• Full Name: iroquois homeobox 6
• Calculated Molecular Weight: 446 aa, 48 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC136573
• Gene Symbol: IRX6
• Gene ID (NCBI): 79190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRX6 is a member of the Iroquois homeobox gene family. Members of this family appear to play multiple roles during pattern formation of vertebrate embryos.