• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IRSp53 Monoclonal antibody, PBS Only

IRSp53 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D4G1

Cat no : 68348-1-PBS

Synonyms

BAI associated protein 2, BAI1 associated protein 2, BAIAP2, BAP2, Fas ligand associated factor 3, FLAF3, Insulin receptor substrate p53, IRS 58, IRSP53, IRSp53/58, Protein BAP2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IRSp53 Monoclonal antibody (68348-1-Ig)

    WB analysis using 68348-1-Ig (same clone as 68348-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68348-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using IRSp53 Monoclonal antibody (68348-1-Ig)

    WB analysis of LNCaP using 68348-1-Ig (same clone as 68348-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68348-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit cerebellum tissue using IRSp53 Monoclonal antibody (68348-1-Ig)

    WB analysis of rabbit cerebellum using 68348-1-Ig (same clone as 68348-1-PBS)

    rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68348-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68348-1-PBS targets IRSp53 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IRSp53 fusion protein Ag25161
Full Name BAI1-associated protein 2
Calculated Molecular Weight 61 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC014020
Gene Symbol IRSp53
Gene ID (NCBI) 10458
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IRSp53 (also known as BAIAP2) is a multi-domain adaptor protein that originally identified as a 58/53-kDa substrate of insulin-receptor kinase in the hamster (PMID: 10332026). As a BAI1-binding protein, IRSp53 plays an important role in linking between membrane and cytoskeleton in the process of neuronal growth (PMID: 10343108). IRSp53 is necessary for Cdc42-mediated reorganization of the actin cytoskeleton and for Rac1-mediated membrane ruffling. Alternatively spliced isoforms of IRSp53 exist. IRSp53 has been implicated in several psychiatric disorders, including autism spectrum disorders (ASDs), schizophrenia, and attention deficit/hyperactivity disorder (ADHD) (PMID: 26275848).