IRSp53 Monoclonal antibody, PBS Only

IRSp53 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D4G1

Cat no : 68348-1-PBS

Synonyms

BAI associated protein 2, BAI1 associated protein 2, BAIAP2, BAP2, Fas ligand associated factor 3, FLAF3, Insulin receptor substrate p53, IRS 58, IRSP53, IRSp53/58, Protein BAP2

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Validation Data Gallery

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  WB analysis using 68348-1-Ig (same clone as 68348-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68348-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 68348-1-Ig (same clone as 68348-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68348-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit cerebellum using 68348-1-Ig (same clone as 68348-1-PBS)

  rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68348-1-PBS in a different storage buffer formulation.

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Product Information

68348-1-PBS targets IRSp53 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IRSp53 fusion protein Ag25161
• Full Name: BAI1-associated protein 2
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC014020
• Gene Symbol: IRSp53
• Gene ID (NCBI): 10458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IRSp53 (also known as BAIAP2) is a multi-domain adaptor protein that originally identified as a 58/53-kDa substrate of insulin-receptor kinase in the hamster (PMID: 10332026). As a BAI1-binding protein, IRSp53 plays an important role in linking between membrane and cytoskeleton in the process of neuronal growth (PMID: 10343108). IRSp53 is necessary for Cdc42-mediated reorganization of the actin cytoskeleton and for Rac1-mediated membrane ruffling. Alternatively spliced isoforms of IRSp53 exist. IRSp53 has been implicated in several psychiatric disorders, including autism spectrum disorders (ASDs), schizophrenia, and attention deficit/hyperactivity disorder (ADHD) (PMID: 26275848).