IRSp53 Monoclonal antibody

• 

  WB analysis using 68348-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 68348-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit cerebellum using 68348-1-Ig

  rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

IRSp53 Monoclonal Antibody for WB, ELISA

Cat No. 68348-1-Ig

Clone No.1D4G1

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit

Applications

WB, ELISA

Synonyms

BAI associated protein 2, BAI1 associated protein 2, BAIAP2, BAP2, Fas ligand associated factor 3, FLAF3, Insulin receptor substrate p53, IRS 58, IRSP53, IRSp53/58, Protein BAP2

---

Tested Applications

• Positive WB detected in: rabbit brain tissue, LNCaP cells, rabbit cerebellum tissue, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68348-1-Ig targets IRSp53 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IRSp53 fusion protein Ag25161
• Full Name: BAI1-associated protein 2
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC014020
• Gene Symbol: IRSp53
• Gene ID (NCBI): 10458
• RRID: AB_3085071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UQB8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRSp53 (also known as BAIAP2) is a multi-domain adaptor protein that originally identified as a 58/53-kDa substrate of insulin-receptor kinase in the hamster (PMID: 10332026). As a BAI1-binding protein, IRSp53 plays an important role in linking between membrane and cytoskeleton in the process of neuronal growth (PMID: 10343108). IRSp53 is necessary for Cdc42-mediated reorganization of the actin cytoskeleton and for Rac1-mediated membrane ruffling. Alternatively spliced isoforms of IRSp53 exist. IRSp53 has been implicated in several psychiatric disorders, including autism spectrum disorders (ASDs), schizophrenia, and attention deficit/hyperactivity disorder (ADHD) (PMID: 26275848).