IRE1; ERN1 Polyclonal antibody, PBS Only

• 

  WB analysis using 28164-1-AP (same clone as 28164-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 28164-1-AP (IRE1; ERN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28164-1-PBS in a different storage buffer formulation.

IRE1; ERN1 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 28164-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

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Product Information

28164-1-PBS targets IRE1; ERN1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRE1; ERN1 fusion protein Ag27375
• Full Name: endoplasmic reticulum to nucleus signaling 1
• Calculated Molecular Weight: 977 aa, 110 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC130405
• Gene Symbol: IRE1
• Gene ID (NCBI): 2081
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75460
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)