IRE1; ERN1 Polyclonal antibody

• 

  WB analysis using 28164-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 28164-1-AP (IRE1; ERN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IRE1; ERN1 Polyclonal Antibody for WB, ELISA

Cat No. 28164-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

ERN1, FLJ30999, hIRE1p, Inositol requiring protein 1, IRE1, Ire1 alpha, IRE1; ERN1, IRE1P

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Tested Applications

• Positive WB detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28164-1-AP targets IRE1; ERN1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRE1; ERN1 fusion protein Ag27375
• Full Name: endoplasmic reticulum to nucleus signaling 1
• Calculated Molecular Weight: 977 aa, 110 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC130405
• Gene Symbol: IRE1
• Gene ID (NCBI): 2081
• RRID: AB_3086033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75460
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)

Publications

Species	Application	Title
human	WB	J Inflamm Res; A Neutrophil Extracellular Traps-Related Gene Trait Revealed the Prospective Therapy Strategy of Coronary Atherosclerosis; Authors - Zhetao Li