IRE1; ERN1 Polyclonal antibody, PBS Only

IRE1; ERN1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27528-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 27528-1-AP (same clone as 27528-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27528-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 27528-1-AP (same clone as 27528-1-PBS)

  IP result of anti-IRE1; ERN1 (IP:27528-1-AP, 4ug; Detection:27528-1-AP 1:500) with A549 cells lysate 600 ug. This data was developed using the same antibody clone with 27528-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 27528-1-AP (same clone as 27528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27528-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 27528-1-AP (same clone as 27528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27528-1-AP (IRE1; ERN1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27528-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 27528-1-AP (same clone as 27528-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human IRE1; ERN1 (27528-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27528-1-PBS in a different storage buffer formulation.

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Product Information

27528-1-PBS targets IRE1; ERN1 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRE1; ERN1 fusion protein Ag25580
• Full Name: endoplasmic reticulum to nucleus signaling 1
• Calculated Molecular Weight: 977 aa, 110 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC130405
• Gene Symbol: IRE1
• Gene ID (NCBI): 2081
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)