IRE1; ERN1 Polyclonal antibody
IRE1; ERN1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF, FC (Intra), IP, ELISA
Conjugate
Unconjugated
103
Cat no : 27528-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, LNCaP cells, PC-3 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 96 publications below |
| IHC | See 8 publications below |
| IF | See 7 publications below |
Product Information
27528-1-AP targets IRE1; ERN1 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IRE1; ERN1 fusion protein Ag25580 |
| Full Name | endoplasmic reticulum to nucleus signaling 1 |
| Calculated Molecular Weight | 977 aa, 110 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC130405 |
| Gene Symbol | IRE1 |
| Gene ID (NCBI) | 2081 |
| RRID | AB_2880899 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IRE1; ERN1 antibody 27528-1-AP | Download protocol |
| IHC protocol for IRE1; ERN1 antibody 27528-1-AP | Download protocol |
| IP protocol for IRE1; ERN1 antibody 27528-1-AP | Download protocol |
| FC protocol for IRE1; ERN1 antibody 27528-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. | ||
Cell Stem Cell Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis. | ||
Redox Biol FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response | ||
Genome Biol Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes | ||
J Hazard Mater Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells. | ||
Redox Biol Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor. |
