INTS5 Recombinant antibody, PBS Only (Detector)

INTS5 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230462B10

Cat no : 83154-1-PBS

Synonyms

INTS 5, Int5, 230462B10

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Validation Data Gallery

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  WB analysis using 83154-1-RR (same clone as 83154-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83154-1-RR (INTS5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83154-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83154-1-RR (same clone as 83154-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS5 antibody (83154-1-RR, Clone: 230462B10 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83154-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83154-1-RR (same clone as 83154-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug INTS5 Recombinant antibody (83154-1-RR, Clone:230462B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83154-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00141-2

  Cytometric bead array standard curve ofMP00141-2, INTS5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83154-3-PBS. Detection antibody: 83154-1-PBS. Standard: Ag5129. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83154-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83154-1-RR against Human INTS5 were performed. The affinity constant is 0.968 nM.

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Product Information

83154-1-PBS targets INTS5 as part of a matched antibody pair:

MP00141-2: 83154-3-PBS capture and 83154-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: INTS5 fusion protein Ag5129
• Full Name: integrator complex subunit 5
• Calculated Molecular Weight: 108 kDa
• GenBank Accession Number: BC060841
• Gene Symbol: INTS5
• Gene ID (NCBI): 80789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.