INPP5E Polyclonal antibody, PBS Only

• 

  WB analysis using 29353-1-AP (same clone as 29353-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29353-1-AP (INPP5E antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29353-1-PBS in a different storage buffer formulation.

• 

  IF Staining of ARPE-19 using 29353-1-AP (same clone as 29353-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29353-1-PBS in a different storage buffer formulation.

• 

  IF Staining of ARPE-19 using 29353-1-AP (same clone as 29353-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29353-1-PBS in a different storage buffer formulation.

INPP5E Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 29353-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

---

Product Information

29353-1-PBS targets INPP5E in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INPP5E fusion protein Ag30305
• Full Name: inositol polyphosphate-5-phosphatase, 72 kDa
• Calculated Molecular Weight: 644 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC028032
• Gene Symbol: INPP5E
• Gene ID (NCBI): 56623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRR6
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.