INPP5E Polyclonal antibody

INPP5E Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29353-1-AP

Synonyms

INPP5E, PPI5PIV

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Validation Data Gallery

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  WB analysis using 29353-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29353-1-AP (INPP5E antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of ARPE-19 using 29353-1-AP

  Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of ARPE-19 using 29353-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using INPP5E antibody (29353-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue, rat testis tissue
• Positive IF/ICC detected in: ARPE-19 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29353-1-AP targets INPP5E in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INPP5E fusion protein Ag30305
• Full Name: inositol polyphosphate-5-phosphatase, 72 kDa
• Calculated Molecular Weight: 644 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC028032
• Gene Symbol: INPP5E
• Gene ID (NCBI): 56623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215). It has 2 isoforms with the molecular mass of 66 kDa and 70 kDa.