INPP4B Recombinant antibody, PBS Only (Detector)

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  WB analysis using 85151-4-RR (same clone as 85151-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85151-4-RR (INPP4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85151-4-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 85151-4-RR (same clone as 85151-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using INPP4B antibody (85151-4-RR, Clone: 242837H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85151-4-PBS in a different storage buffer formulation.

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  FC experiment of K562 using 85151-4-RR (same clone as 85151-4-PBS)

  1x10^6 K562 were intracellularly stained with 0.25 ug INPP4B Recombinant antibody (85151-4-RR, Clone:242837H2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85151-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01878-1

  Cytometric bead array standard curve of MP01878-1, INPP4B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85151-3-PBS. Detection antibody: 85151-4-PBS. Standard: Ag8676. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 85151-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85151-4-RR against Human INPP4B were performed. The affinity constant is 0.563 nM.

INPP4B {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 85151-4-PBS

Clone No.242837H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

242837H2, EC:3.1.3.66, Inositol polyphosphate 4-phosphatase type II, Type II inositol 3,4-bisphosphate 4-phosphatase

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Product Information

85151-4-PBS targets INPP4B as part of a matched antibody pair:

MP01878-1: 85151-3-PBS capture and 85151-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: INPP4B fusion protein Ag8676
• Full Name: inositol polyphosphate-4-phosphatase, type II, 105kDa
• Calculated Molecular Weight: 53 aa, 6 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC005273
• Gene Symbol: INPP4B
• Gene ID (NCBI): 8821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15327
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

INPP4B (Inositol polyphosphate 4-phosphatase type II) can catalyze the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate (PMID: 24070612; 24591580). It also Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (PMID: 24591580).