INO80 Monoclonal antibody, PBS Only

INO80 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F11C5

Cat no : 68064-1-PBS

Synonyms

hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259

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Validation Data Gallery

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  WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68064-1-PBS targets INO80 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: INO80 fusion protein Ag20265
• Full Name: INO80 homolog (S. cerevisiae)
• Calculated Molecular Weight: 1556 aa, 177 kDa
• Observed Molecular Weight: 177 kDa
• GenBank Accession Number: BC146785
• Gene Symbol: INO80
• Gene ID (NCBI): 54617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.