CoraLite® Plus 488-conjugated INF2 Monoclonal antibody

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  IF Staining of HepG2 using CL488-66910

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 INF2 antibody (CL488-66910, Clone: 4A11A7 ) at dilution of 1:200.

INF2 Monoclonal Antibody for IF/ICC

Cat No. CL488-66910

Clone No.4A11A7

Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

IF/ICC

Synonyms

C14orf151, C14orf173, DKFZp762A0214, FLJ22056, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-66910 targets INF2 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: INF2 fusion protein Ag17043
• Full Name: inverted formin, FH2 and WH2 domain containing
• Calculated Molecular Weight: 1249 aa, 136 kDa
• Observed Molecular Weight: 150-170 kDa
• GenBank Accession Number: BC008756
• Gene Symbol: INF2
• Gene ID (NCBI): 64423
• RRID: AB_2919386
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q27J81
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).

Publications

Species	Application	Title
mouse	IF	Kidney360; AMPK-SP1-Guided Dynein Expression Represents a New Energy-Responsive Mechanism and Therapeutic Target for Diabetic Nephropathy; Authors - Jillian Williquett