CoraLite® Plus 488-conjugated INF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

INF2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20466

Synonyms

C14orf151, C14orf173, DKFZp762A0214, FLJ22056, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-20466

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 INF2 antibody (CL488-20466) at dilution of 1:200. DAPI (blue).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-20466 is INF2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INF2 fusion protein Ag14302
• Full Name: inverted formin, FH2 and WH2 domain containing
• Calculated Molecular Weight: 1249 aa, 136 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: BC008756
• Gene Symbol: INF2
• Gene ID (NCBI): 64423
• RRID: AB_2919171
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).