CoraLite®594-conjugated INA Monoclonal antibody

INA Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C12A8

Cat no : CL594-67120

Synonyms

Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin

---

Validation Data Gallery

• 

  IF Staining of SH-SY5Y using CL594-67120

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite®594 INA antibody (CL594-67120, Clone: 3C12A8 ) at dilution of 1:200.

• 

  FC experiment of SH-SY5Y using CL594-67120

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human INA (CL594-67120, Clone:3C12A8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: SH-SY5Y cells
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67120 targets INA in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: INA fusion protein Ag13655
• Full Name: internexin neuronal intermediate filament protein, alpha
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: BC006359
• Gene Symbol: INA
• Gene ID (NCBI): 9118
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

INA, also named as Alpha internexin, is a 66 kDa intermediate filament protein which is involved in the morphogenesis of neurons. INA is mainly expressed in various kinds of central and peripheral neurons from early development and highly expressed in neurodegenerative and neuronal death cells. The MW of INA ranges from 60 kDa in human, pig and mouse to 66 kDa in rat.(PMID: 29940892, 2201753)