CoraLite®594-conjugated IMPDH2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IMPDH2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-12948

Synonyms

IMPDH II, IMPDH 2, IMPD2, IMPD 2, IMP dehydrogenase II

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Validation Data Gallery

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  IF Staining of HeLa using CL594-12948

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 IMPDH2 antibody (CL594-12948) at dilution of 1:100, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).

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  IF Staining of HeLa using CL594-12948

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 IMPDH2 antibody (CL594-12948) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-12948 targets IMPDH2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IMPDH2 fusion protein Ag3632
• Full Name: IMP (inosine monophosphate) dehydrogenase 2
• Calculated Molecular Weight: 514 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC012840
• Gene Symbol: IMPDH2
• Gene ID (NCBI): 3615
• RRID: AB_2919791
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) IMPDH2 is highly enriched in RR(filamentous rods and rings). This antibody may has cross reaction with IMPDH1 due to the high homology.