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IL7 Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG1

Detection: Mouse / IgG2b

Reactivity

Capture: Human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 3.125-100 ng/mL

Cat No : MP50135-1

Print datasheet

Synonyms

IL 7, IL7, interleukin 7

Validation Data Gallery

  • Cytometric bead array standard curve of MP50135-1, IL7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60206-2-PBS. Detection antibody: 60206-1-PBS. Standard:ag16314. Range: 3.125-100 ng/mL.

    Cytometric bead array standard curve of MP50135-1

    Cytometric bead array standard curve of MP50135-1, IL7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60206-2-PBS. Detection antibody: 60206-1-PBS. Standard:ag16314. Range: 3.125-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP50135-1 targets IL7 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 3.125-100 ng/mL (Cytometric Bead Array);

Capture antibody: IL-7 Monoclonal antibody, PBS Only (Capture) 60206-2-PBS (4B3C5). 100 μg. Concentration 1 mgl/ml.

Detection antibody: IL-7 Monoclonal antibody, PBS Only (Detector) 60206-1-PBS (5D5G7). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard:

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 60206-2-PBS
Tested Reactivity Human
Immunogen IL-7 fusion protein Ag16314
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=8.51 x 10-10M
KOff=7.03 x 10-6M
KOn=8.26 x 103M
Full Name interleukin 7
Synonyms IL 7, IL7, IL-7, interleukin 7
GenBank accession numberBC047698
Gene symbol IL7
UniprotP13232
Gene ID (NCBI) 3574
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 60206-1-PBS
Tested Reactivity human
Immunogen IL-7 fusion protein Ag16314
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Affinity
KD=7.40 x 10-10M
KOff=7.82 x 10-6M
KOn=1.06 x 104M
Full Name interleukin 7
Synonyms IL 7, IL7, IL-7, interleukin 7
GenBank accession numberBC047698
Gene symbol IL7
UniprotP13232
Gene ID (NCBI) 3574
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.