CD126/IL-6R alpha Recombinant antibody, PBS Only

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  WB analysis of RAW 264.7 using 83688-4-RR (same clone as 83688-4-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83688-4-RR (CD126/IL-6R alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83688-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83688-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83688-4-RR against Human CD126/IL-6R alpha were performed. The affinity constant is 0.80 nM.

CD126/IL-6R alpha Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83688-4-PBS

Clone No.240734D1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

CD126, IL-6R alpha, 240734D1, IL 6R alpha, IL 6R 1

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Product Information

83688-4-PBS targets CD126/IL-6R alpha in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 6 receptor
• Calculated Molecular Weight: 468 aa, 52 kDa
• Observed Molecular Weight: 65 kDA
• GenBank Accession Number: BC132684
• Gene Symbol: IL-6R alpha
• Gene ID (NCBI): 3570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P08887
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892).