CD126/IL-6R alpha Recombinant antibody

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  WB analysis of RAW 264.7 using 83688-4-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83688-4-RR (CD126/IL-6R alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83688-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83688-4-RR against Human CD126/IL-6R alpha were performed. The affinity constant is 0.80 nM.

CD126/IL-6R alpha Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83688-4-RR

Clone No.240734D1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

CD126, IL-6R alpha, 240734D1, IL 6R alpha, IL 6R 1

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Tested Applications

• Positive WB detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83688-4-RR targets CD126/IL-6R alpha in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 6 receptor
• Calculated Molecular Weight: 468 aa, 52 kDa
• Observed Molecular Weight: 65 kDA
• GenBank Accession Number: BC132684
• Gene Symbol: IL-6R alpha
• Gene ID (NCBI): 3570
• RRID: AB_3671290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P08887
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892).