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IL-4 Recombinant antibody, PBS Only (Capture)

IL-4 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240794A8

Cat no : 83894-1-PBS

Synonyms

IL4, interleukin 4, IL 4, BSF-1, B-cell stimulatory factor 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using IL-4 Recombinant antibody (83894-1-RR)

    IF Staining of Ramos using 83894-1-RR (same clone as 83894-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using IL-4 antibody (83894-1-RR, Clone: 240794A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83894-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00829-1

    Cytometric bead array standard curve of MP00829-1

    Cytometric bead array standard curve of MP00829-1, IL-4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83894-1-PBS. Detection antibody: 83894-4-PBS. Standard: Eg0104. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83894-1-RR

    Affinity and Kinetic Characterization of 83894-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83894-1-RR against Human IL-4 were performed. The affinity constant is 1.41 nM.

Product Information

83894-1-PBS targets IL-4 as part of a matched antibody pair:

MP00829-1: 83894-1-PBS capture and 83894-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 4
Calculated Molecular Weight 153 aa, 17 kDa
GenBank Accession NumberBC070123
Gene Symbol IL-4
Gene ID (NCBI) 3565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.