IL-4 Recombinant antibody

IL-4 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240794A8

Cat no : 83894-1-RR

Synonyms

IL4, interleukin 4, IL 4, BSF-1, B-cell stimulatory factor 1

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Validation Data Gallery

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  IF Staining of Ramos using 83894-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using IL-4 antibody (83894-1-RR, Clone: 240794A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83894-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83894-1-RR against Human IL-4 were performed. The affinity constant is 1.41 nM.

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Tested Applications

• Positive IF/ICC detected in: Ramos cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83894-1-RR is IL-4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 4
• Calculated Molecular Weight: 153 aa, 17 kDa
• GenBank Accession Number: BC070123
• Gene Symbol: IL-4
• Gene ID (NCBI): 3565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.