IL-27 Monoclonal antibody, PBS Only

IL-27 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1H5

Cat no : 66164-1-PBS

Synonyms

IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha

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Validation Data Gallery

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  WB analysis of Recombinant protein using 66164-1-Ig (same clone as 66164-1-PBS)

  IL27 fusion protein were subjected to SDS PAGE followed by western blot with 66164-1-Ig (IL-27 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66164-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66164-1-Ig (same clone as 66164-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66164-1-Ig (IL-27 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66164-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66164-1-Ig (same clone as 66164-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66164-1-Ig (IL-27 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66164-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66164-1-PBS targets IL-27 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-27 fusion protein Ag21436
• Full Name: interleukin 27
• Calculated Molecular Weight: 243 aa, 27 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC062422
• Gene Symbol: IL-27
• Gene ID (NCBI): 246778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IL-27, as a member of the IL-6/IL-12 family, is a heterodimeric cytokine composed of two subunits: p28 (IL-27a) and EBI3 (IL-27b). IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.