IL-21R Monoclonal antibody

IL-21R Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1C2

Cat no : 66319-1-Ig

Synonyms

IL21R, IL-21 receptor, IL 21R, IL 21 receptor, CD360

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Validation Data Gallery

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  WB analysis using 66319-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL-21R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66319-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL-21R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66319-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL-21R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66319-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66319-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, A172 cells, MJ cells, K-562 cells, TF-1 cells, MOLT-4 cells, HuT 78 cells, NK-92 cells, human placenta tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66319-1-Ig targets IL-21R in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-21R fusion protein Ag21295
• Full Name: interleukin 21 receptor
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC004348
• Gene Symbol: IL-21R
• Gene ID (NCBI): 50615
• RRID: AB_2881700
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL21R, a receptor for interleukin-21, belongs to the type I cytokine receptor family. IL21R has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.