IL-21R Monoclonal antibody, PBS Only

IL-21R Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D1C2

Cat no : 66319-1-PBS

Synonyms

CD360, IL 21 receptor, IL 21R, IL21R, IL-21R, interleukin 21 receptor, NILR, Novel interleukin receptor

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Validation Data Gallery

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  WB analysis using 66319-1-Ig (same clone as 66319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL-21R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66319-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66319-1-Ig (same clone as 66319-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL-21R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66319-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66319-1-Ig (same clone as 66319-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66319-1-Ig (IL21R Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66319-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66319-1-Ig (same clone as 66319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66319-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66319-1-Ig (same clone as 66319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66319-1-Ig (IL21R antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66319-1-PBS in a different storage buffer formulation.

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Product Information

66319-1-PBS targets IL-21R in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-21R fusion protein Ag21295
• Full Name: interleukin 21 receptor
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC004348
• Gene Symbol: IL-21R
• Gene ID (NCBI): 50615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IL21R, a receptor for interleukin-21, belongs to the type I cytokine receptor family. IL21R has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.