Rat IL2 Recombinant antibody, PBS Only (Capture)

IL2 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240264B2

Cat no : 82626-2-PBS

Synonyms

interleukin 2, IL-2, 240264B2

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00535-3

  Sandwich ELISA standard curve of MP00535-3, rat IL-2 Recombinant Matched Antibody Pair - PBS only. 82626-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0260. 82626-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP00535-3

  Rat spleen, cut into 1-2 mm pieces, was cultured for 3-4 daysin 25-30 mL of RPMIsupplemented with 10% fetal bovine serum and stimulated with 10 μg/mL ConA. The cell culture supernate wasremoved, assayed for rat IL-2, and measured 45,596.05 pg/mL.

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  Sample test of MP00535-3

  Serum of seven rats was measured. The IL-2 concentration of detected samples was determined to be 95.71 pg/mL with a range of ND - 179.2 pg/mL.

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  Cytometric bead array standard curve of MP00535-1

  Cytometric bead array standard curve of MP00535-1, RAT IL-2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82626-2-PBS. Detection antibody: 82626-3-PBS. Standard: Eg0260. Range: 0.156-20 ng/mL.

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  Cytometric bead array standard curve of MP00535-2

  Cytometric bead array standard curve of MP00535-2, RAT IL-2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82626-2-PBS. Detection antibody: 82626-4-PBS. Standard: Eg0260. Range: 0.156-20 ng/mL.

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Product Information

82626-2-PBS targets IL2 as part of a matched antibody pair:

MP00535-1: 82626-2-PBS capture and 82626-3-PBS detection (validated in Cytometric bead array)

MP00535-2: 82626-2-PBS capture and 82626-4-PBS detection (validated in Cytometric bead array)

MP00535-3: 82626-2-PBS capture and 82626-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 2
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_053836
• Gene Symbol: Il2
• Gene ID (NCBI): 116562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.