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Rat IL-2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: rat

Detection: rat

Applications

Sandwich ELISA

Range

Sandwich ELISA: 15.6-1000 pg/mL

Cat No : MP00535-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00535-3, rat IL-2 Recombinant Matched Antibody Pair - PBS only. 82626-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0260. 82626-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

    Sandwich ELISA standard curve of MP00535-3

    Sandwich ELISA standard curve of MP00535-3, rat IL-2 Recombinant Matched Antibody Pair - PBS only. 82626-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0260. 82626-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Serum of seven rats was measured. The IL-2 concentration of detected samples was determined to be 95.71 pg/mL with a range of ND - 179.2 pg/mL.

    Sample test of MP00535-3

    Serum of seven rats was measured. The IL-2 concentration of detected samples was determined to be 95.71 pg/mL with a range of ND - 179.2 pg/mL.

  • Rat spleen, cut into 1-2 mm pieces, was cultured for 3-4 daysin 25-30 mL of RPMIsupplemented with 10% fetal bovine serum and stimulated with 10 μg/mL ConA. The cell culture supernate wasremoved, assayed for rat IL-2, and measured 45,596.05 pg/mL.

    Sample test of MP00535-3

    Rat spleen, cut into 1-2 mm pieces, was cultured for 3-4 daysin 25-30 mL of RPMIsupplemented with 10% fetal bovine serum and stimulated with 10 μg/mL ConA. The cell culture supernate wasremoved, assayed for rat IL-2, and measured 45,596.05 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00535-3 targets IL-2 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: Rat IL2 Recombinant antibody, PBS Only (Capture) 82626-2-PBS (240264B2). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Rat IL2 Recombinant antibody, PBS Only (Detector) 82626-5-PBS (240264F4). 100 μg. Concentration 1 mgl/ml.

Alternative IL-2 matched antibody pairs: MP00533-1, MP00533-2, MP00533-3, MP00535-2, MP00545-1, MP00545-2, MP00545-3

Matched pair protein standard: Eg0260

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 82626-2-PBS
Tested Reactivity rat
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 2
Synonyms interleukin 2, IL-2, 240264B2
GenBank accession numberNM_053836
Gene symbol Il2
UniprotP17108
Gene ID (NCBI) 116562
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 82626-5-PBS
Tested Reactivity rat
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 2
Synonyms interleukin 2, IL-2, 240264F4
GenBank accession numberNM_053836
Gene symbol Il2
UniprotP17108
Gene ID (NCBI) 116562
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.