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Rat IL2 Recombinant antibody, PBS Only (Detector)

IL2 Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240264F4

Cat no : 82626-5-PBS

Synonyms

interleukin 2, IL-2, 240264F4

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00535-3

    Sandwich ELISA standard curve of MP00535-3

    Sandwich ELISA standard curve of MP00535-3, rat IL-2 Recombinant Matched Antibody Pair - PBS only. 82626-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0260. 82626-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00535-3

    Sample test of MP00535-3

    Rat spleen, cut into 1-2 mm pieces, was cultured for 3-4 daysin 25-30 mL of RPMIsupplemented with 10% fetal bovine serum and stimulated with 10 μg/mL ConA. The cell culture supernate wasremoved, assayed for rat IL-2, and measured 45,596.05 pg/mL.

  • Sample test of MP00535-3

    Sample test of MP00535-3

    Serum of seven rats was measured. The IL-2 concentration of detected samples was determined to be 95.71 pg/mL with a range of ND - 179.2 pg/mL.

Product Information

82626-5-PBS targets IL2 as part of a matched antibody pair:

MP00535-3: 82626-2-PBS capture and 82626-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 2
Calculated Molecular Weight18 kDa
GenBank Accession NumberNM_053836
Gene Symbol Il2
Gene ID (NCBI) 116562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.