CoraLite®594-conjugated IL-1R2 Monoclonal antibody

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  IF Staining of HeLa using CL594-60262

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated IL1R2 antibody (CL594-60262, Clone: 3H4H4 ) at dilution of 1:100.

IL-1R2 Monoclonal Antibody for IF/ICC

Cat No. CL594-60262

Clone No.3H4H4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1R2, IL1RB, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60262 targets IL-1R2 in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4495

  Product name: Recombinant human IL-1R2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 24-347 aa of BC039031

  Sequence: AARSCRFRGRHYKREFRLEGEPVALRCPQVPYWLWASVSPRINLTWHKNDSARTVPGEEETRMWAQDGALWLLPALQEDSGTYVCTTRNASYCDKMSIELRVFENTDAFLPFISYPQILTLSTSGVLVCPDLSEFTRDKTDVKIQWYKDSLLLDKDNEKFLSVRGTTHLLVHDVALEDAGYYRCVLTFAHEGQQYNITRSIELRIKKKKEETIPVIISPLKTISASLGSRLTIPCKVFLGTGTPLTTMLWWTANDTHIESAYPGGRVTEGPRQEYSENNENYIEVPLIFDPVTREDLHMDFKCVVHNTLSFQTLRTTVKEASST

• Full Name: interleukin 1 receptor, type II
• Calculated Molecular Weight: 398 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC039031
• Gene Symbol: IL1R2
• Gene ID (NCBI): 7850
• RRID: AB_2883451
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P27930
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Interleukin-1 receptor type 2 (IL1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. It exists in both a membrane bound and soluble form. IL1R2 binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 serves as a negative regulator of IL-1 signaling, thereby suppressing inflammatory response.