IL-1 beta Recombinant antibody, PBS Only (Capture/Detector)

IL-1 beta Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

2G9

Cat no : 82696-8-PBS

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00331-2

  Sandwich ELISA standard curve of MP00331-2, Human IL-1 beta Recombinant Matched Antibody Pair - PBS only. 82696-15-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0286. 82696-8-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

• 

  Sandwich ELISA standard curve of MP80001-6

  Sandwich ELISA standard curve of MP80001-6, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-8-RR. Detection antibody: HRP-conjugated 66737-1-PBS. Standard:HZ-1164. Range: 0.098-12.5 ng/mL.

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  Sample test of MP00331-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA or 10 μg/mL PHA and 10 ng/mL IL-2 for 1 day. The mean IL-1 beta concentration was not determined in unstimulated PBMC supernatant, 5,709.3 pg/mL in PHA stimulated PBMC supernatant and 1,110.8 pg/mL in PHA and IL-2 stimulated PBMC supernatant.

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  Sample test of MP00331-2

  Serum of four individual healthy human donors was measured. The IL-1 beta concentration of detected samples was determined to be 93.4 pg/mL with a range of 20.3 - 131.0 pg/mL.

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  Cytometric bead array standard curve of MP00331-2

  Cytometric bead array standard curve of MP00331-2, IL-1 beta Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82696-15-PBS. Detection antibody: 82696-8-PBS. Standard: Eg0286. Range: 0.313-40 ng/mL.

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Product Information

82696-8-PBS targets IL-1 beta as part of a matched antibody pair:

MP00331-2: 82696-15-PBS capture and 82696-8-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP80001-6: 82696-8-PBS capture and 66737-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 1, beta
• Calculated Molecular Weight: 269 aa, 31 kDa
• GenBank Accession Number: BC008678
• Gene Symbol: IL1B
• Gene ID (NCBI): 3553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.