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IL-12/IL-23 p40 Monoclonal antibody, PBS Only

IL-12/IL-23 p40 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B2A8

Cat no : 66288-1-PBS

Synonyms

IL12B, IL 12B, IL 12 subunit p40, IL 12 P40, Cytotoxic lymphocyte maturation factor 40 kDa subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using IL-12/IL-23 p40 Monoclonal antibody (66288-1-Ig)

    WB analysis of human liver using 66288-1-Ig (same clone as 66288-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 66288-1-Ig (IL12B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66288-1-PBS in a different storage buffer formulation.

Product Information

66288-1-PBS targets IL-12/IL-23 p40 in WB, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-12/IL-23 p40 fusion protein Ag21429
Full Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Calculated Molecular Weight 328 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC067498
Gene Symbol IL12B
Gene ID (NCBI) 3593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).