IL-11 Monoclonal antibody, PBS Only (Capture)

IL-11 Monoclonal Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

1B3H6

Cat no : 68135-2-PBS

Synonyms

IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP50054-1

  Sandwich ELISA standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair - PBS only. 68135-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag11199. 68135-1-PBS was biotinylated as the detection antibody. Range: 6.25-400 pg/mL.

• 

  Sample test of MP50054-1

  Cytometric bead array of Saos-2 cells supernatant using MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Saos-2 cells were inoculated into 6 well plate at 40000 cells/well followed by culture for 3 days in normal culture media or HGF (Cat.NO. HZ-1084, 500 ng/mL) containing media. 50 μL of the supernatant from each day was analyzed using MP50054-1 (IL11 Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the stimulation time. Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

• 

  Cytometric bead array standard curve of MP50054-1

  Cytometric bead array standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Standard:Ag11199. Range: 1.563-100 ng/mL.

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Product Information

68135-2-PBS targets IL-11 as part of a matched antibody pair:

MP50054-1: 68135-2-PBS capture and 68135-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=1.30 x 10^-9M
  K_Off=6.38 x 10^-6M
  K_On=4.89 x 10^3M
• Immunogen: IL-11 fusion protein Ag11199
• Full Name: interleukin 11
• Calculated Molecular Weight: 199 aa, 21 kDa
• GenBank Accession Number: BC012506
• Gene Symbol: IL-11
• Gene ID (NCBI): 3589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.