IL-6 Recombinant antibody, PBS Only

IL-6 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240670F10

Cat no : 83747-5-PBS

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

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Validation Data Gallery

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  WB analysis of HUVEC using 83747-5-RR (same clone as 83747-5-PBS)

  LPS and Brefeldin A treated HUVEC cells were subjected to SDS PAGE followed by western blot with 83747-5-RR (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83747-5-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 83747-5-RR (same clone as 83747-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IL-6 antibody (83747-5-RR, Clone: 240670F10 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83747-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83747-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83747-5-RR against Human IL-6 were performed. The affinity constant is 1.60 nM.

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Product Information

The immunogen of 83747-5-PBS is IL-6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 6 (interferon, beta 2)
• Calculated Molecular Weight: 212 aa, 24 kDa
• GenBank Accession Number: BC015511
• Gene Symbol: IL6
• Gene ID (NCBI): 3569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.