Anti-Human IL-4RA/CD124 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98203-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-4RA/CD124 Rabbit Recombinant Antibody (98203-1-RR, Clone:241824D8) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98203-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-4RA/CD124 Rabbit Recombinant Antibody (98203-1-RR, Clone:241824D8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

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  Affinity and Kinetic Characterization of 98203-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98203-1-RR against Human IL-4RA/CD124 were performed. The affinity constant is 53.6 pM.

IL-4RA/CD124 Recombinant Antibody for FC

Cat No. 98203-1-RR

Clone No.241824D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98203-1-RR targets IL-4RA/CD124 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 4 receptor
• Calculated Molecular Weight: 90 kDa
• GenBank Accession Number: NM_000418.4
• Gene Symbol: IL-4R
• Gene ID (NCBI): 3566
• RRID: AB_3672335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P24394-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.