IL-4 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

IL-4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G2C5

Cat no : 66142-1-PBS

Synonyms

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra, PS1TP5BP1

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Validation Data Gallery

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  WB analysis of Jurkat using 66142-1-Ig (same clone as 66142-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66142-1-Ig (IL-4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66142-1-Ig (same clone as 66142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig (IL-4 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66142-1-Ig (same clone as 66142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66142-1-Ig (IL-4 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66142-1-Ig (same clone as 66142-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-4 antibody (66142-1-Ig, Clone: 1G2C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66142-1-Ig (same clone as 66142-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-4 antibody (66142-1-Ig, Clone: 1G2C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Ramos using 66142-1-Ig (same clone as 66142-1-PBS)

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human IL-4 (66142-1-Ig, Clone:1G2C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66142-1-PBS in a different storage buffer formulation.

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Product Information

66142-1-PBS targets IL-4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-4 fusion protein Ag9940
• Full Name: interleukin 4
• Calculated Molecular Weight: 153 aa, 17 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC070123
• Gene Symbol: IL-4
• Gene ID (NCBI): 3565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.