Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody

IL-2RG/CD132 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

242020H6

Cat no : 98234-1-RR

Synonyms

IL2RG, IL 2RG, IL 2R subunit gamma, IL 2 receptor subunit gamma, gammaC

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Validation Data Gallery

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  FC experiment of human PBMCs using 98234-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody (98234-1-RR, Clone: 242020H6), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD4. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98234-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody (98234-1-RR, Clone: 242020H6)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98234-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98234-1-RR against Human IL-2RG/CD132 were performed. The affinity constant is 1.55 nM.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98234-1-RR targets IL-2RG/CD132 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 2 receptor, gamma (severe combined immunodeficiency)
• Calculated Molecular Weight: NM_000206.3
• GenBank Accession Number: NM_000206.3
• Gene Symbol: IL2RG
• Gene ID (NCBI): 3561
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL-2RA), beta (IL-2RB), and gamma (IL-2RG). IL-2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL-2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.