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Tested Applications
| Positive FC (Intra) detected in | PMA, Ionomycin and Brefeldin A treated human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-98053 targets IL-2 in FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | IL-2 fusion protein Eg0828 Predict reactive species |
| Full Name | interleukin 2 |
| Calculated Molecular Weight | 153 aa, 18 kDa |
| GenBank Accession Number | BC070338 |
| Gene Symbol | IL2 |
| Gene ID (NCBI) | 3558 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P60568 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus IL-2 antibody FITC-98053 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
