CoraLite® Plus 488-conjugated IL-2 Monoclonal antibody

IL-2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F11C5

Cat no : CL488-60306

Synonyms

Aldesleukin, IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-60306

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 IL-2 antibody (CL488-60306, Clone: 2F11C5 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL488-60306

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 IL-2 antibody (CL488-60306, Clone: 2F11C5 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60306 targets IL-2 in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-2 fusion protein Ag10199
• Full Name: interleukin 2
• Calculated Molecular Weight: 153 aa, 18 kDa
• GenBank Accession Number: BC070338
• Gene Symbol: IL2
• Gene ID (NCBI): 3558
• RRID: AB_2919247
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.