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Mouse IL-2 Recombinant antibody, PBS Only (Detector)

IL-2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240358B1

Cat no : 83547-3-PBS

Synonyms

Il2, Interleukin-2, interleukin 2, Il 2, 240358B1

Validation Data Gallery

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  • Western Blot (WB) analysis of EL-4 cells using IL-2 Recombinant antibody (83547-3-RR)

    WB analysis of EL-4 using 83547-3-RR (same clone as 83547-3-PBS)

    Untreated, PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 83547-3-RR (IL-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83547-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00533-2

    Cytometric bead array standard curve of MP00533-2

    Cytometric bead array standard curve of MP00533-2, MOUSE IL-2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83547-2-PBS. Detection antibody: 83547-3-PBS. Standard: Eg0762. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83547-3-RR

    Affinity and Kinetic Characterization of 83547-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83547-3-RR against Mouse IL-2 were performed. The affinity constant is 36.5 nM.

Product Information

83547-3-PBS targets IL-2 as part of a matched antibody pair:

MP00533-2: 83547-2-PBS capture and 83547-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 2
Calculated Molecular Weight 19 kDa
Observed Molecular Weight15 kDa
GenBank Accession Number NM_008366
Gene Symbol IL-2
Gene ID (NCBI) 16183
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-2 (Il2) is a protein initially identified as a T cell growth factor. Il2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors. Nevertheless, it has been shown that Il2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (Il2R). Il2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.