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IL-2 Recombinant antibody

IL-2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240358B1

Cat no : 83547-3-RR

Synonyms

Il2, Interleukin-2, interleukin 2, Il 2, 240358B1

Validation Data Gallery

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  • Western Blot (WB) analysis of EL-4 cells using IL-2 Recombinant antibody (83547-3-RR)

    WB analysis of EL-4 using 83547-3-RR

    Untreated, PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 83547-3-RR (IL-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83547-3-RR

    Affinity and Kinetic Characterization of 83547-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83547-3-RR against Mouse IL-2 were performed. The affinity constant is 36.5 nM.

Tested Applications

Positive WB detected inPMA(50ng/ml)+iono(500ng/ml)共6h换液+Bref A 18h EL-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83547-3-RR is IL-2 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 2
Calculated Molecular Weight 19 kDa
Observed Molecular Weight15 kDa
GenBank Accession Number NM_008366
Gene Symbol IL-2
Gene ID (NCBI) 16183
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-2 (Il2) is a protein initially identified as a T cell growth factor. Il2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors. Nevertheless, it has been shown that Il2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (Il2R). Il2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.

Protocols

Product Specific Protocols
WB protocol for IL-2 antibody 83547-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols