1X10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-1F10 (CL488-60319, Clone:5B2F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-1F10 (CL488-60319, Clone:5B2F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
Background Information
IL1F10 is a member of the interleukin 1 cytokine family. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.
Protocols
Product Specific Protocols
FC protocol for CL Plus 488 IL-1F10 antibody CL488-60319
1X10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-1F10 (CL488-60319, Clone:5B2F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IL-1F10 Monoclonal antibody
Catalog Number
CL488-60319
Citations
-
Dilutions
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
FC (Intra)
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
