IL-1F10 Monoclonal antibody

IL-1F10 Monoclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

5B2F5

Cat no : 60319-1-Ig

Synonyms

FIL1 theta, FIL1T, FKSG75, IL 1 theta, IL 1F10, IL 1HY2, IL1 theta, IL1F10, IL-1F10, IL1HY2, IL38, Interleukin 1 family member 10, Interleukin 1 HY2, Interleukin 1 theta, UNQ6119/PRO20041

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Validation Data Gallery

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  WB analysis of Recombinant protein using 60319-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60319-1-Ig (IL1F10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of U-937 using 60319-1-Ig

  1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human IL-1F10 (60319-1-Ig, Clone:5B2F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive FC (Intra) detected in: U-937 cells
• Positive FC detected in: U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60319-1-Ig targets IL-1F10 in WB, ELISA, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-1F10 fusion protein Ag17250
• Full Name: interleukin 1 family, member 10 (theta)
• Calculated Molecular Weight: 152 aa, 17 kDa
• GenBank Accession Number: BC103966
• Gene Symbol: IL1F10
• Gene ID (NCBI): 84639
• RRID: AB_2881430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL1F10 is a member of the interleukin 1 cytokine family. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.