CoraLite® Plus 647 Anti-Mouse IL-17F Rabbit Recombinant Antibody

IL-17F Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

230026G6

Cat no : CL647-98019

Synonyms

Il17f, interleukin 17F, IL 17F, C87042, 230026G6

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Validation Data Gallery

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  FC experiment of C57BL/6 Th17-polarized splenocytes using CL647-98019

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th17-polarized splenocytes were intracellularly stained with 0.25ug CoraLite® Plus 647 Anti-Mouse IL-17F Rabbit Recombinant Antibody (CL647-98019, Clone:230026G6). Cells were then stained with FITC Plus Anti-Mouse CD4. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Tested Applications

• Positive FC (Intra) detected in: PMA, Ionomycin and Brefeldin A treated C57BL/6 Th17-polarized splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98019 is IL-17F Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 17F
• Calculated Molecular Weight: 17KD
• GenBank Accession Number: NM_145856.2
• Gene Symbol: IL-17F
• Gene ID (NCBI): 257630
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

The interleukin 17 (IL-17) family of cytokines contains 6 structurally related cytokines, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 family plays crucial roles in host defense against microbial organisms and in the development of inflammatory diseases. IL-17A is a pro-inflammatory cytokine that also has the capacity to promote angiogenesis and osteoclastogenesis. IL-17F shares the highest homology with IL-17A and signals via a receptor composed by the IL-17RA and IL-17RC subunits. IL-17A and IL-17F can form IL-17A/A or IL-17F/F homodimers, IL-17A/F heterodimers are also formed. IL-17A and IL-17F, produced by the Th17 CD4(+) T cell lineage, have been linked to a variety of inflammatory and autoimmune conditions. IL-17F levels are elevated in sera and lesional psoriatic skin compared to non-lesional tissue. IL-17F also has been implicated in the development of neutrophilic airway inflammation.